01773nas a2200181   4500008004100000022001400041245016000055210006900215260000800284300001400292490000800306520108200314100001801396700001901414700001801433700001501451856012501466       2025                        eng d  a1520-610600aMolecular modeling of non-canonical intramolecular RNA triple helix structures predicted from TRIPinRNA and their in vitro biophysical structure validation0 aMolecular modeling of noncanonical intramolecular RNA triple hel  cAPR  a4298-43080 v1293 a<p>
	RNA triple helices have traditionally been characterized by pyrimidine-type UA-U or CG-C triplets, with other base triplets considered to be destabilizing. However, the presence of non-canonical triplets in riboswitches and self-splicing introns suggests that triplexes containing longer stretches of such triplets may exist in the human genome too. Using molecular modeling, we investigated a chimeric triple helix derived from the FLRT2-AS1 lncRNA, confirming its stability over a 500 ns simulation. Biophysical analyses further support the formation of this triplex in vitro. Although these non-canonical structures exhibit less thermal stability compared to traditional UA-U triplets found in lncRNAs like metastasis associated lung adenocarcinoma transcript 1 and NEAT1, they may serve distinct biological functions, suggesting a dynamic and more temporal role in cellular processes. The triplex selected for this study is found in a human long non-coding RNA gene, paving the way for investigating the intriguing roles of these triple helices in cell biology.</p>
1 aRakheja, Isha1 aPanda, Gayatri1 aMaiti, Souvik1 aRay, Arjun  uhttps://library.ncl.res.in/content/molecular-modeling-non-canonical-intramolecular-rna-triple-helix-structures-predicted