02517nas a2200241   4500008004100000022001400041245011500055210006900170260000800239300000700247490000700254520170100261653002401962653001401986653003202000653001102032653002902043653001602072100002002088700001602108700002202124856012902146       2025                        eng d  a2191-085500aAspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel production0 aAspergillus terreus variant TB21 wet biomass optimized by insitu  cFEB  a230 v153 a<p>
	The oleaginous fungus, Aspergillus terreus when subjected to random chemical mutagenesis led to isolation of TB21 variant with improved lipid content (78.1%) as compared to wild type (49.8%). The fungal wet biomass grown on sugarcane bagasse hydrolysate (SCBH) was subjected to one-step in-situ (direct) acid transesterification to optimize its conversion to biodiesel using a 2-level factorial statistical design of experiments. The process optimization revealed that wet biomass and methanol were the most significant factors and in a short reaction time period of 5 min with low methanol: wet biomass ratio (10:1) influenced FAME production Statistical optimization studies showed that TB21 exhibited a higher FAME content of 76.5 and 38.1% (w/w) from wet and dry biomass, respectively when compared to wild type (48.1 and 24.5%). FAME productivity (0.55-1 h-1) and a yield (66 gL-1) were achieved when TB21 was grown on SCBH for 120 h at 30 degrees C. The FAME profile from the wet biomass of TB21 grown on SCBH had desirable amounts of saturated (77.7%), monounsaturated (7.2%), and polyunsaturated (2.4%) methyl esters. Physico-chemical properties of TB21-derived biodiesel were determined, namely, density(0.88 g cm-3), kinematic viscosity (4.1 mm s-2), iodine value (96.82), cetane number (55.31), free fatty acid content (0.15%), total acid number (0.3 NaOH mg g-1), meeting international (ASTM D6751, EN 14214) and Indian (IS 15607) standards. Thus, the direct one-pot in situ transesterification reaction using wet biomass of variant TB21 strain showed improved production and quality of biodiesel with potential large scale application using the low-cost substrate (SCBH).</p>
10aAspergillus Terreus10aBiodiesel10aIn situ transesterification10aMutant10aStatistical optimization10aWet biomass1 aBed, Rashmi, K.1 aKumar, Ravi1 aRaviKumar, Ameeta  uhttps://library.ncl.res.in/content/aspergillus-terreus-variant-tb21-wet-biomass-optimized-situ-transesterification-biodiesel