02358nas a2200193 4500008004100000245013700041210007100178260000800249490000700257520161900264100002301883700002001906700002501926700001701951700002301968700002501991700001802016856013002034 2025 eng d00aOxidase-like nanozyme activity of ultrathin copper metal–organic framework nanosheets with high specificity for catechol oxidation0 aOxidaselike nanozyme activity of ultrathin copper metal–organic  cJAN0 v173 a

In nature, catechol oxidase catalyzes the oxidation of o-diphenol to o-quinone to produce a series of highly important polyphenolic natural products. Although mimicking the functionality of natural enzyme using a nanozyme was found to be beneficial, attaining a high specificity is challenging. Herein, we have explored the thickness-dependent oxidase activity and specificity of Cu-metal-organic framework (MOF) nanosheets. The unique synthetic method offers control over the thickness of the Cu-MOF nanosheets. The ultrathin (4 nm) Cu-MOF (Cu-UMOF) nanosheets as an oxidase nanozyme exhibit high specificity for catechol oxidation without having any peroxidase activity. Interestingly, the thicker (20–30 nm) Cu-MOF nanosheets showed poor catechol oxidase and peroxidase activity. The binuclear Cu-center, coordinative, and electronic unsaturation tuned electronic structure in Cu-UMOF, resulting in higher specificity for catechol oxidation than thicker Cu-MOF.

1 aSingh, Ajit, Kumar1 aSharma, Deepika1 aSingh, Devesh, Kumar1 aSarraf, Sonu1 aBasu, Aviru, Kumar1 aGanesan, Vellaichamy1 aSaha, Avishek uhttps://library.ncl.res.in/content/oxidase-nanozyme-activity-ultrathin-copper-metal%E2%80%93organic-framework-nanosheets-high