02552nas a2200229   4500008004100000022001400041245015300055210006900208260000900277300001400286490000600300520168500306100002601991700002302017700002202040700002302062700002002085700003802105700002102143700002802164856013002192       2024                        eng d  a2470-134300aPoly(<sc>d</sc>,<sc>l</sc>-lactide-<i>co</i>-glycolide) surface-anchored biotin-loaded irinotecan nanoparticles for active targeting of colon cancer0 aPolyscdscsclsclactideicoiglycolide surfaceanchored biotinloaded   cJAN   a3807-38260 v93 a<p>
	A poly-(d,l-lactide-co-glycolide) (PLGA) copolymer was synthesized using the ring-opening polymerization of d,l-lactide and glycolide monomers in the presence of zinc proline complex in bulk through the green route and was well characterized using attenuated total reflectance-Fourier transform infrared, H-1 and C-13 nuclear magnetic resonance, gel permeation chromatography, differential scanning calorimetry, X-ray diffraction, matrix-assisted laser desorption/ionization time-of-flight, etc. Furthermore, PLGA-conjugated biotin (PLGA-B) was synthesized using the synthesized PLGA and was employed to fabricate nanoparticles for irinotecan (Ir) delivery. These nanoparticles (PLGA-NP-Ir and PLGA-B-NP-Ir) were tested for physicochemical and biological characteristics. PLGA-B-NP-Ir exhibited a stronger cellular uptake and anticancer activity as compared to PLGA-NP-Ir in CT-26 cancer cells (log p &lt; 0.05). The accumulation and retention of fluorescence-labeled nanoparticles were observed to be better in CT-26-inoculated solid tumors in Balb/c mice. The PLGA-B-NP-Ir-treated group inhibited tumor growth significantly more (log p &lt; 0.001) than the untreated control, PLGA-NP-Ir, and Ir-treated groups. Furthermore, no body weight loss, hematological, and blood biochemical tests demonstrated the nanocarriers' nontoxic nature. This work presents the use of safe PLGA and the demonstration of a proof-of-concept of biotin surface attached PLGA nanoparticle-mediated active targeted Ir administration to combat colon cancer. To treat colon cancer, PLGA-B-NP-Ir performed better due to specific active tumor targeting and greater cellular uptake due to biotin.</p>
1 aGiram, Prabhanjan, S.1 aNimma, Ramakrishna1 aBulbule, Anuradha1 aYadav, Amit, Singh1 aGorain, Mahadeo1 aRadharani, Nalukurthi, Naga Venka1 aKundu, Gopal, C.1 aGarnaik, Baijayantimala  uhttps://library.ncl.res.in/content/polydl-lactide-co-glycolide-surface-anchored-biotin-loaded-irinotecan-nanoparticles-active