02156nas a2200229   4500008004100000022001400041245010800055210006900163260000900232300000700241490000700248520134100255653002701596653001701623653003401640653003301674653002401707100002601731700001601757700002401773856012901797       2023                        eng d  a2191-085500aPotential of Y. lipolytica epoxide hydrolase for efficient production of enantiopure (R)-1,2-octanediol0 aPotential of Y lipolytica epoxide hydrolase for efficient produc  cJUL   a770 v133 a<p>
	The recombinant Yleh from a tropical marine yeast Yarrowia lipolytica NCIM 3589 exhibited a high epoxide hydrolase activity of 9.34 +/- 1.80 mu mol min(-1) mg(-1) protein towards 1,2-epoxyoctane (EO), at pH 8.0 and 30 degrees C. The reaction product was identified as 1,2-Octanediol (OD) by GC-MS using EO and H2O18 as substrate, affirming the functionality of Yleh as an epoxide hydrolase. For EO, the K-m, V-max, and k(cat)/K-m values were 0.43 +/- 0.017 mM, 0.042 +/- 0.003 mM min(-1), and 467.17 +/- 39.43 mM(-1) min(-1), respectively. To optimize the reaction conditions for conversion of racemic EO by Yleh catalyst to enantiopure (R)-1,2-octanediol, initially, Response Surface Methodology was employed. Under optimized reaction conditions of 15 mM EO, 150 mu g purified Yleh at 30 degrees C a maximal diol production of 7.11 mM was attained in a short span of 65 min with a yield of 47.4%. Green technology using deep eutectic solvents for the hydrophobic substrate (EO) were tested as co-solvents in Yleh catalyzed EO hydrolysis. Choline chloride-Glycerol, produced 9.08 mM OD with an increased OD yield of 60.5%. Thus, results showed that deep eutectic solvents could be a promising solvent for Yleh-catalyzed reactions making Yleh a potential biocatalyst for the biosynthesis of enantiopure synthons. [GRAPHICS] .</p>
10aDeep Eutectic solvents10aOptimization10aRecombinant epoxide hydrolase10aResponse surface methodology10aYarrowia lipolytica1 aGodase, Vijaya, P. P.1 aKumar, Ravi1 aKumar, Ameeta, Ravi  uhttps://library.ncl.res.in/content/potential-y-lipolytica-epoxide-hydrolase-efficient-production-enantiopure-r-12-octanediol