02096nas a2200265   4500008004100000022001400041245011000055210006900165260000800234300001400242490000700256520118500263100001801448700002701466700002801493700001601521700002701537700003101564700001901595700001801614700002401632700002701656700001801683856012901701       2023                        eng d  a0305-104800aIdentification of G-quadruplex structures in MALAT1 lncRNA that interact with nucleolin and nucleophosmin0 aIdentification of Gquadruplex structures in MALAT1 lncRNA that i  cSEP  a9415-94310 v513 a<p>
	Nuclear-retained long non-coding RNAs (lncRNAs) including MALAT1 have emerged as critical regulators of many molecular processes including transcription, alternative splicing and chromatin organization. Here, we report the presence of three conserved and thermodynamically stable RNA G-quadruplexes (rG4s) located in the 3 &amp; PRIME; region of MALAT1. Using rG4 domain-specific RNA pull-down followed by mass spectrometry and RNA immunoprecipitation, we demonstrated that the MALAT1 rG4 structures are specifically bound by two nucleolar proteins, Nucleolin (NCL) and Nucleophosmin (NPM). Using imaging, we found that the MALAT1 rG4s facilitate the localization of both NCL and NPM to nuclear speckles, and specific G-to-A mutations that disrupt the rG4 structures compromised the localization of both NCL and NPM in speckles. In vitro biophysical studies established that a truncated version of NCL (&amp; UDelta;NCL) binds tightly to all three rG4s. Overall, our study revealed new rG4s within MALAT1, established that they are specifically recognized by NCL and NPM, and showed that disrupting the rG4s abolished localization of these proteins to nuclear speckles</p>
1 aGhosh, Arpita1 aPandey, Satya, Prakash1 aJoshi, Dheeraj, Chandra1 aRana, Priya1 aAnsari, Asgar, Hussain1 aSundar, Jennifer, Seematti1 aSingh, Praveen1 aKhan, Yasmeen1 aEkka, Mary, Krishna1 aChakraborty, Debojyoti1 aMaiti, Souvik  uhttps://library.ncl.res.in/content/identification-g-quadruplex-structures-malat1-lncrna-interact-nucleolin-and-nucleophosmin