01910nas a2200169   4500008004100000022001400041245007500055210006900130260000900199300001400208490000700222520133700229100002701566700002001593700002001613856010701633       2023                        eng d  a1744-683X00aDifferential membrane curvature induced by distinct protein conformers0 aDifferential membrane curvature induced by distinct protein conf  cJUN   a4021-40280 v193 a<p>
	Membrane topology changes are associated with various cellular processes and are modulated by synergistic effects between lipid composition and membrane-associated proteins. However, how protein shape or conformational dynamics couples to membrane molecular properties remains unclear. In this work, we aim to investigate this coupling behavior using the curvature-inducing protein caveolin-1. We considered distinct protein conformers of the helical hairpin protein corresponding to different protein shapes, such as the wedge and the banana-shaped conformers. The different protein conformers were simulated in a coarse-grain representation in the presence of cholesterol-sphingomyelin rich membrane. We observed that membrane curvature is dependent on protein shape and is the lowest for the wedge conformer and maximal for the banana conformer. The differences in the net stress between the two membrane leaflets, calculated from the lateral pressure profile distributions in lipid bilayers for different protein conformers, show a similar trend. In conjunction, we show that cholesterol and sphingomyelin clustering in the membrane is modulated by protein shape. Overall, our results provide molecular-level insights into the coupling between membrane topology, protein shape and lipid clustering in cell membranes.</p>
1 aMalshikare, Hrushikesh1 aPrakash, Shikha1 aSengupta, Durba  uhttps://library.ncl.res.in/content/differential-membrane-curvature-induced-distinct-protein-conformers