02384nas a2200253   4500008004100000022001400041245011400055210006900169260000800238300001000246490000700256520154000263653002001803653002001823653001301843653001501856653001501871100002101886700002001907700003001927700002201957700002601979856012502005       2022                        eng d  a1075-261700aTryptophan-cardanol fluorescent nanoparticles inhibit alpha-synuclein aggregation and disrupt amyloid fibrils0 aTryptophancardanol fluorescent nanoparticles inhibit alphasynucl  cAPR  ae33740 v283 a<p>
	Protein misfolding and aggregation play a vital role in several human diseases such as Parkinson's, Alzheimer's, and prion diseases. The development of nanoparticles that modulate aggregation could be potential drug candidates for these neurodegenerative disorders. Parkinson's disease pathogenesis is closely associated with the accumulation of alpha-synuclein oligomers and fibrils in the substantia nigra of the brain. This report discusses the interactions of novel tryptophan-cardanol nanoparticles with alpha-synuclein protein monomers and fibrils. These nanoparticles could effectively disrupt alpha-synuclein fibrils and inhibit fibril formation at low concentrations such as 5 mu M. The tryptophan-cardanol nanoparticles inhibit fibril formation from unstructured protein resulting in spherical nanostructures. These nanoparticles could also disassemble amyloid fibrils; the complete disappearance of fibrils was evident after 48 h of incubation with tryptophan-cardanol. The transmission electron microscopy (TEM) micrographs after the incubation did not show any remnants of the peptide aggregates or oligomers. The thioflavin T fluorescence after the disassembly was diminished compared with that of fibrils also supports the inhibitory effect of the nanoparticles. Also, these nanoparticles did not reduce the viability of the SH-SY5Y cells. These findings suggest that the tryptophan-cardanol nanoparticles showed sufficiently high inhibitory activity and may have therapeutic potential for synucleinopathies.</p>
10aalpha-synuclein10aamyloid fibrils10aCardanol10ainhibitors10aTryptophan1 aSunny, Lisni, P.1 aSrikanth, Priya1 aSunitha, Anju, Kunhiraman1 aTembulkar, Niyoti1 aAbraham, Jancy, Nixon  uhttps://library.ncl.res.in/content/tryptophan-cardanol-fluorescent-nanoparticles-inhibit-alpha-synuclein-aggregation-and