02463nas a2200241   4500008004100000022001400041245019400055210006900249260000800318300000800326490000800334520156100342653002601903653001901929653001501948653002901963653001401992100001802006700001502024700002802039700002602067856012802093       2022                        eng d  a0302-893300aIsoeugenol affects expression pattern of conidial hydrophobin gene RodA and transcriptional regulators MedA and SomA responsible for adherence and biofilm formation in Aspergillus fumigatus0 aIsoeugenol affects expression pattern of conidial hydrophobin ge  cAPR  a2140 v2043 a<p>
	Aspergillus fumigatus is one of the major pathogenic fungal species, causing life-threatening infections. Due to a limited spectrum of available antifungals, exploration of new drug targets as well as potential antifungal molecules has become pertinent. Rodlet layer plays an important role in adherence of fungal conidia to hydrophobic cell surfaces in host, which also leads to A. fumigatus biofilm formation, contributing factor to fungal pathogenicity. From decades, natural sources have been known for the development of new active molecules. The present study investigates effect of isoeugenol on genes responsible for hydrophobins (RodA), adhesion as well as biofilm formation (MedA and SomA) of A. fumigatus. Minimum inhibitory concentrations (MIC and IC50) of isoeugenol against A. fumigatus were determined using broth microdilution assay. The IC50 results showed reduced hydrophobicity and biofilm formation as well as eradication after treatment with the compound and electron micrograph data corroborated these findings. The qRT-PCR showed a significant downregulation of genes RodA, MedA, SomA and pksP involved in hydrophobicity and biofilm formation. SwissADME studies potentiated drug-like propensity for isoeugenol which formed four hydrogen bonds with low binding energy (- 4.54 kcal/mol) at the catalytic site of RodA protein studied via AutoDock4. Hence, the findings conclude that isoeugenol inhibits conidial hydrophobicity and biofilm formation of A. fumigatus and further investigations are warranted in this direction.</p>
10aAspergillus fumigatus10aHydrophobicity10aIsoeugenol10aTranscription regulators10aVirulence1 aGupta, Lovely1 aSen, Pooja1 aBhattacharya, Asish, K.1 aVijayaraghavan, Pooja  uhttps://library.ncl.res.in/content/isoeugenol-affects-expression-pattern-conidial-hydrophobin-gene-roda-and-transcriptional