02298nas a2200265   4500008004100000022001400041245012000055210006900175260000800244300000800252490000700260520143500267653001201702653002401714653002001738653001701758653001701775100001801792700001901810700002301829700001901852700002201871700001901893856012001912       2022                        eng d  a2190-572X00aMetagenomic mining of Indian river confluence reveal functional microbial community with lignocelluloytic potential0 aMetagenomic mining of Indian river confluence reveal functional   cJUN  a1320 v123 a<p>
	Microbial carbohydrate-active enzymes (CAZyme) can be harnessed for valorization of Lignocellulosic biomass (LCB) to value-added chemicals/products. The two Indian Rivers Ganges and the Yamuna having different origins and flow, face accumulation of carbon-rich substrates due to the discharge of wastewater from adjoining paper and pulp industries, which could potentially contribute to the natural enrichment of LCB utilizing genes, especially at their confluence. We analyzed CAZyme diversity in metagenomic datasets across the sacred confluence of the Rivers Ganges and Yamuna. Functional annotation using CAZyme database identified a total of 77,815 putative genes with functional domains involved in the catalysis of carbohydrate degradation or synthesis of glycosidic bonds. The metagenomic analysis detected similar to 41% CAZymes catalyzing the hydrolysis of lignocellulosic biomass polymers- cellulose, hemicellulose, lignin, and pectin. The Beta diversity analysis suggested higher CAZyme diversity at downstream region of the river confluence, which could be useful niche for culture-based studies. Taxonomic origin for CAZymes revealed the predominance of bacteria (97%), followed by archaea (1.67%), Eukaryota (0.63%), and viruses (0.7%). Metagenome guided CAZyme diversity of the microflora spanning across the confluence of Ganges-Yamuna River, could be harnessed for biomass and bioenergy applications.</p>
10aCAZymes10aConfluence (Sangam)10aLignocellulosic10aRiver Ganges10aRiver Yamuna1 aRajput, Vinay1 aSamson, Rachel1 aYadav, Rakeshkumar1 aDastager, Syed1 aKhairnar, Krishna1 aDharne, Mahesh  uhttps://library.ncl.res.in/content/metagenomic-mining-indian-river-confluence-reveal-functional-microbial-community