02285nas a2200277   4500008004100000022001400041245011300055210006900168260000800237300001200245490000800257520137100265653002801636653002101664653001001685653002801695653002501723100002001748700001901768700001801787700002001805700001701825700002201842700002001864856012301884       2022                        eng d  a0141-813000aSuperior processability of Antheraea mylitta silk with cryo-milling: performance in bone tissue regeneration0 aSuperior processability of Antheraea mylitta silk with cryomilli  cJUL  a155-1650 v2133 a<p>
	Non-mulberry silk polymers have a promising future in biomedical applications. However, the dissolution of nonmulberry silk fiber is a still challenge and this poor processability has limited the use of this material. Here, we report a unique protocol to process the Antheraea mylitta (AM) silk fiber. We have shown that the cryo-milling of silk fiber reduces the beta sheet content by more than 10% and results in an SF powder that completely dissolves in routine solvents like trifluoroacetic acid (TFA) within few hours to form highly concentrated solutions (\~20 wt %). Further, these solutions can be processed using conventional processing techniques such as electrospinning to form 3D scaffolds. Bombyx mori (BM) silk was used as a control sample in the study. In-vitro studies were also performed to monitor cell adhesion and proliferation and hMSCs differentiation into osteogenic lineage. Finally, the osteogenic potential of the scaffolds was also evaluated by a 4-week implantation study in rat calvarial model. The in-vitro and in-vivo results show that the processing techniques do not affect the biocompatibility of the material and the AM scaffolds support bone regeneration. Our results, thus, show that cryo-milling facilitates enhanced processability of non-mulberry silk and therefore expands its potential in biomedical applications.</p>
10aBone tissue engineering10aCalvarial defect10ahMSCs10aNon-mulberry silk fiber10aProcessing technique1 aParekh, Nimisha1 aBijosh, C., K.1 aKane, Kartiki1 aPanicker, Alaka1 aNisal, Anuya1 aWangikar, Pralhad1 aAgawane, Sachin  uhttps://library.ncl.res.in/content/superior-processability-antheraea-mylitta-silk-cryo-milling-performance-bone-tissue