02458nas a2200277   4500008004100000020001300041245007900054210006900133260000800202300000900210490000700219520159500226653002501821653002001846653001401866653003001880653002601910653002601936100002201962700002001984700001802004700002202022700002102044700001702065856009802082       2021                        eng d  a0971119800aGreen antibacterial molecules: sophorolipids with varying fatty acid chain0 aGreen antibacterial molecules sophorolipids with varying fatty a  cNOV  a431+0 v353 aAntibiotic resistance is a rising problem of over the last decade making it difficult to treat infections caused by various pathogenic bacteria. To tackle this problem, newer antimicrobial strategies are being explored. Sophorolipids (SLs) are a class of biomolecules produced by a non-pathogenic yeast Starmarella bombicola (S. bombicold) with surfactant as well as potent broad spectrum antibacterial activity. In this work, we have successfully synthesized sophorolipids using different fatty acids with increasing chain length of hydrophobic tail; Lauric acid (C12)-LASL, Myristic acid (C14)-MASL, Palmitic acid (C16)-PASL and Stearic acid (C18)-SASL. We have used various techniques like FTIR, HRMS to characterize the synthesized SL. Further, we have measured properties like the critical micellar concentration for these biosurfactants and the SL's showed CMC less than or equal to 100 mg/L indicating excellent surface-active property. We have studied the antibacterial activity against gram-negative and gram-positive bacteria. Interestingly, the antibacterial activity was found to be more potent as the chain length of the fatty acid in the SL decreased. This trend was found to be reverse for toxicity towards mammalian cells. The shorter chain fatty acid SL's were found to be less cytotoxic. These results indicate that the sophorolipid candidates with shorter chain lengths would be beneficial for various biomedical applications such as tissue engineering, regenerative medicine and wound healing. Keywords: sophorolipid, biosurfactant, short chain fatty acid, antibacterial10aAntibacterial agents10aCells (Biology)10aInfection10aMicrobial drug resistance10aSaturated fatty acids10aSurface active agents1 aHirlekar, Swarali1 aAbhyankar, Isha1 aKane, Kartiki1 aTrimukhe, Kalpana1 aPrabhune, Asmita1 aNisal, Anuya  uhttps://link.gale.com/apps/doc/A684660702/AONE?u=anon~d0cd1b4e&sid=googleScholar&xid=1a6d61d9