01427nas a2200229   4500008004100000022001400041245012300055210006900178260000900247300001600256490000600272520062400278653001300902653001800915653002700933653001900960653002300979100002001002700002101022700002401043856013001067       2021                        eng d  a2365-654900aReplacement of loop residues in TBA by an abasic ethylene glycol spacer: effect on stability, structure and function**0 aReplacement of loop residues in TBA by an abasic ethylene glycol  cOCT   a10648-106500 v63 aThis article describes the synthesis of ethyleneglycol (E) phosphoramidite and its incorporation into the thrombin binding aptamer (TBA) sequence at loop positions. Circular dichroism (CD) study revealed no major disturbances in the secondary structure of TBA by the abasic E unit and the derived oligomers exhibited a typical antiparallel chair-like conformation similar to that of TBA. UV and CD spectroscopy, together with anti-coagulation and HPLC studies revealed that although nuclease stability was enhanced, and anti-coagulation reasonably good, the thermal stability of the quadruplexes was adversely affected.10aaptamers10aclotting time10aethylene glycol spacer10aG-quadruplexes10anuclease stability1 aWagh, Atish, A.1 aGhalawat, Monika1 aFernandes, Moneesha  uhttps://library.ncl.res.in/content/replacement-loop-residues-tba-abasic-ethylene-glycol-spacer-effect-stability-structure-and