02199nas a2200241   4500008004100000022001400041245009100055210006900146260000800215300001200223490000800235520139800243653003201641653001801673653002301691653002401714653002001738653002501758653001701783100001801800700001901818856012001837       2021                        eng d  a1359-511300aTrace metals in cellular metabolism and their impact on recombinant protein production0 aTrace metals in cellular metabolism and their impact on recombin  cNOV  a251-2620 v1103 aReplacement of serum and increasing use of chemically defined media demands optimisation of trace metal components for biomanufacturing applications. Trace metal availability can impact culture performance, productivity and product quality. Several trace metals are cofactors of metabolic and other enzymes, and thus their availability regulates cellular metabolism. Additionally, they can also affect the availability of other trace metals and stability of some medium components. Such factors also need to be considered while formulating trace metal concentrations in the culture medium. Due to their very low concentrations, these components are susceptible to substantial variability arising from contaminants from other raw material and leaching from process equipment and can contribute to process variability. Understanding the role and impact of trace metals will help develop strategies to achieve targeted process parameters and increase process robustness vis-`a-vis any lot-to-lot variability in trace metal concentration in culture medium. This review describes the role of trace metals, particularly manganese, copper and zinc, in central carbon metabolism to aid in understanding the basis of metal-mediated effects on culture performance and provides a comprehensive review of the reported impact of trace metals on CHO cell culture performance and recombinant protein quality.10aChinese hamster ovary cells10aglycosylation10aMedium formulation10aProcess variability10aProduct quality10aRecombinant proteins10aTrace metals1 aPrabhu, Anuja1 aGadgil, Mugdha  uhttps://library.ncl.res.in/content/trace-metals-cellular-metabolism-and-their-impact-recombinant-protein-production