01822nas a2200181   4500008004100000245012200041210006900163260001300232490000700245520113100252100002601383700002501409700002601434700002301460700001501483700002301498856011901521       2019                        eng d00aUltrasound assisted extraction of the polysaccharide from Tuber aestivum and its in vitro anti-hyperglycemic activity0 aUltrasound assisted extraction of the polysaccharide from Tuber   bElsevier0 v203 aTuber aestivum is one of the most popular and delicious truffles in the world economic market and is reported to possess a wide range of biological activities. The total carbohydrate of this truffle constitutes about 5.65% w/w dry weight basis (dwb). The present work compares the yield of the polysaccharide obtained by conventional hot water extraction followed by alcohol precipitation, and ultrasound assisted extraction using low power 150 W probe sonifier. The parameters of ultrasound assisted extraction (amplitude, extraction time, pH, and liquid-solid ratio) from the fruiting body of T. aestivum were optimized using Taguchi orthogonal array and central composite design. The optimal parameters of extraction were a liquid-solid ratio of 75:1 for 15 min at pH 6.5 at an amplitude of 25%, which yielded 68.91 ± 1.54% w/w dwb polysaccharide. The FTIR and SEM characteristics of the polysaccharides obtained by ultrasonic extraction and conventional hot water extraction were similar. Further, the polysaccharides extracted from T. aestivum demonstrated a significant in vitro anti-hyperglycemic activity.1 aMudliyar, Devshri, S.1 aWallenius, Janne, H.1 aBedade, Dattatray, K.1 aSinghal, Rekha, S.1 aMadi, Nuri1 aShamekh, Salem, S.  uhttps://library.ncl.res.in/content/ultrasound-assisted-extraction-polysaccharide-tuber-aestivum-and-its-vitro-anti