02493nas a2200289   4500008004100000245009100041210006900132260000800201300001100209520157300220653003001793653002201823653002101845653002101866653001001887653001901897653001601916653001301932653001701945100002101962700001801983700001902001700002202020700001702042700002602059856011802085       2021                        eng d00aSilk fibroin and ceramic scaffolds: comparative in vitro studies for bone regeneration0 aSilk fibroin and ceramic scaffolds comparative in vitro studies   cAPR  ae102213 a<p>Synthetic bone void fillers based on calcium ceramics are used to fill cavities in the bone and promote bone regeneration. More recently, silk fibroin (SF), a protein polymer obtained from Bombyx mori silkworm, has emerged as a promising material in bone void filling. In this work, we have compared the safety and efficacy of two types of silk fibroin-based bone void fillers with currently used and commercially available ceramic bone void fillers (based on calcium sulphate, beta tricalcium phosphate, and beta tricalcium phosphate with hydroxyapatite). Further, we have also evaluated these two types of SF scaffolds, which have strikingly different structural attributes. The biocompatibility of these scaffolds was comparable as assessed by cytotoxicity assay, cellular adhesion assay, and immunogenic assay. Ability of the scaffolds to support differentiation of human mesenchymal stem cells (hMSCs) into an osteoblastic lineage was also evaluated in an in vitro differentiation experiment using reverse transcriptase polymerase chain reaction analysis. These results revealed that cells cultured on SF scaffolds exhibit higher expression of early to late markers such as Runx2, BMPs, collagen, osterix, osteopontin, and osteocalcin as compared with ceramic-based scaffolds. This observation was further validated by studying the expression of alkaline phosphatase and calcium deposition. We also show that scaffolds made from same material of SF, but characterized by very different pore architectures, have diverse outcome in stem cell differentiation.</p>10abeta tricalcium phosphate10abone regeneration10abone void filler10acalcium sulphate10ahMSCs10ahydroxyapatite10aosteoblasts10ascaffold10aSilk fibroin1 aDeshpande, Rucha1 aShukla, Swati1 aSayyad, Raeesa1 aSalunke, Shalmali1 aNisal, Anuya1 aVenugopalan, Premnath  uhttps://library.ncl.res.in/content/silk-fibroin-and-ceramic-scaffolds-comparative-vitro-studies-bone-regeneration