02571nas a2200241   4500008004100000022001400041245009300055210006900148260000800217300000700225490000700232520178400239653001902023653001802042653001602060653001702076653002102093100002102114700002202135700002502157700002202182856012502204       2021                        eng d  a2190-572X00aGlucosinolate induces transcriptomic and metabolic reprogramming in Helicoverpa armigera0 aGlucosinolate induces transcriptomic and metabolic reprogramming  cJAN  a260 v113 a<p>Glucosinolates protect plants from herbivory. Lepidopteran insects have developed resistance to glucosinolates which is well studied. However, the molecular effects of glucosinolate intake on insects are unexplored. To elucidate this, we performed transcriptomics and metabolomics of sinigrin-fed Helicoverpa armigera. Transcriptomics exhibits significant dysregulation of 2375 transcripts, of which 1575 are upregulated and 800 downregulated. Gene Ontology analysis of differentially expressed genes reveals that key hydrolases, oxidoreductases, and transferases are majorly affected. The negative impact of sinigrin is significant and localized in the endomembrane system and mitochondria. It also disturbs various biological processes such as regulation of protein metabolism and cytoskeletal organization. Furthermore, H. armigera putative myrosinase-like enzymes may catalyze the breakdown of sinigrin to allyl isothiocyanate (AITC). AITC targets the electron transport chain causing oxidative stress. KEGG pathway enrichment shows significant upregulation of oxidative phosphorylation, glutathione metabolism and amino acid metabolism. Activation of these pathways induces glutathione synthesis for sinigrin detoxification. Differential gene expression indicates upregulation of glutathione S-transferase and succinate dehydrogenase suggesting mitochondrial impact. Transcriptomics data correlated with metabolomics show changes in serine, methionine, ornithine, and other metabolite levels. It corroborates well with the transcript alterations supporting the increased glutathione production. Thus, our data suggest that sinigrin generates oxidative stress in H. armigera and insects alter their metabolic wiring to overcome sinigrin-mediated deleterious effects.</p>
10aDetoxification10aGlucosinolate10aGlutathione10amitochondria10aOxidative stress1 aJagdale, Shounak1 aTellis, Meenakshi1 aBarvkar, Vitthal, T.1 aJoshi, Rakesh, S.  uhttps://library.ncl.res.in/content/glucosinolate-induces-transcriptomic-and-metabolic-reprogramming-helicoverpa-armigera