02164nas a2200229   4500008004100000022001400041245012300055210006900178260000800247300001200255490000800267520134900275653002101624653001601645653002601661653002901687653002201716100002401738700002301762700002301785856012601808       2021                        eng d  a0175-759800aMetabolic engineering and synthetic biology for isoprenoid production in Escherichia coli and Saccharomyces cerevisiae0 aMetabolic engineering and synthetic biology for isoprenoid produ  cJAN  a457-4750 v1053 a<p>Isoprenoids, often called terpenoids, are the most abundant and highly diverse family of natural organic compounds. In plants, they play a distinct role in the form of photosynthetic pigments, hormones, electron carrier, structural components of membrane, and defence. Many isoprenoids have useful applications in the pharmaceutical, nutraceutical, and chemical industries. They are synthesized by various isoprenoid synthase enzymes by several consecutive steps. Recent advancement in metabolic engineering and synthetic biology has enabled the production of these isoprenoids in the heterologous host systems like Escherichia coli and Saccharomyces cerevisiae. Both heterologous systems have been engineered for large-scale production of value-added isoprenoids. This review article will provide the detailed description of various approaches used for engineering of methyl-d-erythritol-4-phosphate (MEP) and mevalonate (MVA) pathway for synthesizing isoprene units (C-5) and ultimate production of diverse isoprenoids. The review particularly highlighted the efforts taken for the production of C-5-C-20 isoprenoids by metabolic engineering techniques in E. coli and S. cerevisiae over a decade. The challenges and strategies are also discussed in detail for scale-up and engineering of isoprenoids in the heterologous host systems.</p>
10aEscherichia coli10aIsoprenoids10aMetabolic engineering10aSaccharomyces cerevisiae10asynthetic biology1 aNavale, Govinda, R.1 aDharne, Mahesh, S.1 aShinde, Sandip, S.  uhttps://library.ncl.res.in/content/metabolic-engineering-and-synthetic-biology-isoprenoid-production-escherichia-coli-and