02277nas a2200193   4500008004100000022001400041245009600055210006900151260000800220300001400228490000600242520159700248100002001845700001701865700002501882700001701907700002901924856013001953       2021                        eng d  a2470-134300aMyristic acid derived sophorolipid: efficient synthesis and enhanced antibacterial activity0 aMyristic acid derived sophorolipid efficient synthesis and enhan  cJAN  a1273-12790 v63 a<p>Microbial glycolipids are one of the most interesting alternatives to chemical-based surfactants as they exhibit improved biodegradability and less toxicity. However, their potential has been limited because of specificity of the yeast toward fatty acids having a carbon 16 or carbon 18 chain. This study focuses on sophorolipid (SL) production by the yeast Starmerella bombicola using myristic acid, a medium-chain carbon-14 fatty acid that has not been used as a substrate for SL production. The production was optimized for inoculum size and lipophilic substrate concentration. Furthermore, we also studied the effect of medium-chain fatty acid on yeast cell growth and optimized the process for excellent yield. The myristic acid SL (MASL) so synthesized consisted of mono- and diacetylated forms with preferential glycosylation at the methyl end group, as determined by high-resolution mass spectrometry. Individual congeners of the crude mixture were separated using dry column chromatography and then structurally characterized by mass spectrometry. The synthesized MASL was also shown to have promising surface tension, lowering abilities with a low CMC of 14 mg/L. The SL derived from myristic acid exhibited superior antibacterial activity as compared to SL derived from oleic acid. MASL was also found to be more potent against Gram-positive organisms as compared to Gram-negative organisms. This work, therefore, demonstrates successful synthesis of myristic acid-derived SL and its superior antibacterial activity, establishing a promising future for this biosurfactant.</p>
1 aAbhyankar, Isha1 aSevi, Ganesh1 aPrabhune, Asmita, A.1 aNisal, Anuya1 aBayatigeri, Santhakumari  uhttps://library.ncl.res.in/content/myristic-acid-derived-sophorolipid-efficient-synthesis-and-enhanced-antibacterial-activity