02885nas a2200277   4500008004100000022001400041245018000055210006900235260000800304300001200312490000800324520187500332653002102207653002402228653002702252653002102279653002402300100001702324700001802341700001802359700002202377700001802399700002602417700003202443856013202475       2020                        eng d  a0300-817700aUntargeted metabolomics reveals alterations in metabolites of lipid metabolism and immune pathways in the serum of rats after long-term oral administration of Amalaki rasayana0 aUntargeted metabolomics reveals alterations in metabolites of li  cJAN  a147-1600 v4633 a<p>Amalaki rasayana, a traditional preparation, is widely used by Ayurvedic physicians for the treatment of inflammatory conditions, cardiovascular diseases, and cancer. Metabolic alterations induced by Amalaki rasayana intervention are unknown. We investigated the modulations in serum metabolomic profiles in Wistar rats following long-term oral administration of Amalaki rasayana. Global metabolic profiling was performed of the serum of rats administered with either Amalaki rasayana (AR) or ghee + honey (GH) for 18 months and control animals which were left untreated. Amalaki rasayana components were confirmed from AR extract using HR-LCMS analysis. Significant reductions in prostaglandin J2, 11-dehydrothromboxane B2, and higher levels of reduced glutathione and glycitein metabolites were observed in the serum of AR administered rats compared to the control groups. Eleven different metabolites classified as phospholipids, glycerophospholipids, glucoside derivatives, organic acids, and glycosphingolipid were exclusively observed in the AR administered rats. Pathway analysis suggests that altered metabolites in AR administered rats are those associated with different biochemical pathways of arachidonic acid metabolism, fatty acid metabolism, leukotriene metabolism, G-protein mediated events, phospholipid metabolism, and the immune system. Targeted metabolomics confirmed the presence of gallic acid, ellagic acid, and arachidonic acid components in the AR extract. The known activities of these components can be correlated with the altered metabolic profile following long-term AR administration. AR also activates IGF1R-Akt-Foxo3 signaling axis in heart tissues of rats administered with AR. Our study identifies AR components that induce alterations in lipid metabolism and immune pathways in animals which consume AR for an extended period.</p>
10aAmalaki rasayana10aAyurvedic medicines10aLeukotriene metabolism10aLipid metabolism10aMetabolic profiling1 aKumar, Vikas1 aKumar, Aneesh1 aJoseph, Vinod1 aDan, Vipin, Mohan1 aJaleel, Abdul1 aKumar, T., R. Santhos1 aKartha, Chandrasekharan, C.  uhttps://library.ncl.res.in/content/untargeted-metabolomics-reveals-alterations-metabolites-lipid-metabolism-and-immune-pathways