02265nas a2200277   4500008004100000022001400041245008300055210006900138260000800207300001100215490000800226520139500234653002801629653001501657653002201672653002701694653003101721100001801752700001801770700001701788700002001805700001801825700001601843700001701859856011101876       2020                        eng d  a0269-749100aCharacterisation of hyper tolerant Bacillus firmus L-148 for arsenic oxidation0 aCharacterisation of hyper tolerant Bacillus firmus L148 for arse  cJUN  a1141240 v2613 a<p>Groundwater arsenic pollution causes millions of deaths worldwide. Long term natural and anthropogenic activities have increased arsenic levels in groundwater causing higher threats of arsenic exposure. Arsenic hyper-tolerant Firmicute Bacillus firmus L-148 was isolated from arsenic limiting Lonar lake soil, which tolerated more than 3 M arsenic and could oxidize 75 mM arsenite [As(III)] in 14 days. It oxidized As(III) in presence of heavy metals and had unusual pH optima at 9.2. B. firmus L-148 was studied at the biochemical, protein, genomic and transcript level for understanding its arsenic oxidizing machinery. The proteomic and transcript analysis exhibited the presence of ars and aio operon and supported the inducible nature of ars operon. Robust, hyper-tolerant, fast As(III) oxidizing, least nutrient requiring and multi-metal resistance qualities of the strain were used in microcosm studies for bioremediation. Artificial groundwater mimicking microcosm with 75 mM As(III) was developed. Modulation of carbon source, iron and multi metals affected growth and As(III) oxidation rate. The As(III) oxidation was recorded to be 77% in 15 days in presence of sodium acetate and Fe ions. This microcosm study can be explored for bioremediation of arsenic contaminated water and followed by precipitation using other methods. (C) 2020 Elsevier Ltd. All rights reserved.</p>
10aArsenic hyper-tolerance10aLonar lake10aMicrocosm studies10aMulti-metal resistance10aNext generation sequencing1 aBagade, Aditi1 aNandre, Vinod1 aPaul, Dhiraj1 aPatil, Yugendra1 aSharma, Nisha1 aGiri, Ashok1 aKodam, Kisan  uhttps://library.ncl.res.in/content/characterisation-hyper-tolerant-bacillus-firmus-l-148-arsenic-oxidation