01967nas a2200241   4500008004100000022001400041245008400055210006900139260000900208300001000217490000800227520117000235653002401405653002801429653001401457653002901471653001601500653001601516100001601532700002801548700003601576856011301612       2020                        eng d  a0141-813000aEffect of melatonin on tau aggregation and tau-mediated cell surface morphology0 aEffect of melatonin on tau aggregation and taumediated cell surf  cJUN   a30-390 v1523 a<p>Aggregation of Microtubule-associated protein Tau and its deposition in the form of neurofibrillary tangles (NFTs) is one of the pathological hallmarks of Alzheimer's disease (AD). Tau aggregation inhibition has been targeted in various studies including natural compounds and synthetic small molecules. Here, we have studied neurohormone- Melatonin against in vitro Tau aggregation and observed its effect on membrane topology, tubulin network and Tau phosphorylation in Neuro2A and N9 cell lines. The aggregation and conformation of Tau was determined by ThT fluorescence and CD spectroscopy respectively. The morphology of Tau aggregates in presence and absence of Melatonin was studied by transmission electron microscopy. Melatonin was found to reduce the formation of higher order oligomeric structures without affecting the overall aggregation kinetics of Tau. Melatonin also modulates and helps to maintain membrane morphology, independent on tubulin network as evidenced by FE-SEM and immunofluorescence analysis. Overall, Melatonin administration shows mild anti-aggregation and cytoprotective effects. (C) 2020 Elsevier B.V. All rights reserved.</p>
10aAlzheimer's disease10aCell surface morphology10amelatonin10aPaired helical filaments10atau protein10aTauopathies1 aDas, Rashmi1 aBalmik, Abhishek, Ankur1 aChinnathambi, Subashchandrabose  uhttps://library.ncl.res.in/content/effect-melatonin-tau-aggregation-and-tau-mediated-cell-surface-morphology