02336nas a2200241   4500008004100000022001400041245012900055210006900184260000800253300001200261490000700273520151000280653001601790653001501806653003101821653001701852653001501869100002401884700002101908700002201929700002101951856012201972       2020                        eng d  a1438-165600aSilk fibroin 3D microparticle scaffolds with bioactive ceramics: chemical, mechanical, and osteoregenerative characteristics0 aSilk fibroin 3D microparticle scaffolds with bioactive ceramics   cDEC  a20004580 v223 a<p>Preparation of 3D scaffolds for bone tissue engineering (BTE) is a challenging task as it requires appropriate pore size and porosities, mechanical properties, and controlled bioerosion rate. The focus of this work is the fabrication of silk fibroin (SF) 3D microparticle scaffolds with the incorporation of hydroxyapatite (HA) and calcium sulfate (CaS) as bioceramics. Physicochemical characterization shows approximate to 30% filler loading and approximate to 40% optimum porosity with &gt;100 mu m pore size for these filled/unfilled scaffolds. Nanoindentation studies show improved Young's modulus at microparticle level with the incorporation of bioceramics. SF-HA scaffolds showed three fold increase in Young's modulus, whereas SF-CaS showed two fold increase. In vitro bioerosion study results in early bioerosion with SF-CaS scaffold, whereas prolonged bioerosion with SF-HA scaffold. In vitro osteoregenerative potential is analyzed by estimating alkaline phosphatase (ALP), bone morphogenetic protein-2 (BMP-2), and osteocalcin (OCN). SF CaS supports early stage differentiation while SF 50% HA predominantly supports late stage. The expression of TNF-alpha suggests a reduced risk of immune rejection. This work, therefore, concludes that although SF supports bone tissue regeneration, the choice of bioceramic enhances the applicability in various clinical scenarios by providing a controlled bioerosion rate, tunable speed of osteoregeneration, and improved load bearing capacities.</p>
10abioceramics10abioerosion10aosteogenic differentiation10aSilk fibroin10astem cells1 aParekh, Nimisha, A.1 aDeshpande, Rucha1 aShukla, Swati, G.1 aNisal, Anuya, A.  uhttps://library.ncl.res.in/content/silk-fibroin-3d-microparticle-scaffolds-bioactive-ceramics-chemical-mechanical-and