01945nas a2200169   4500008004100000245010400041210006900145260000800214300001400222490000900236520131200245100002601557700001801583700002201601700002301623856012901646       2017                        eng d00aSystematic comparison of the response properties of protein and RNA mediated gene regulatory motifs0 aSystematic comparison of the response properties of protein and   cJUN  a1235-12450 v13  3 aWe present a framework enabling the dissection of the effects of motif structure (feedback or feedforward), the nature of the controller (RNA or protein), and the regulation mode (transcriptional, post-transcriptional or translational) on the response to a step change in the input. We have used a common model framework for gene expression where both motif structures have an activating input and repressing regulator, with the same set of parameters, to enable a comparison of the responses. We studied the global sensitivity of the system properties, such as steady-state gain, overshoot, peak time, and peak duration, to parameters. We find that, in all motifs, overshoot correlated negatively whereas peak duration varied concavely with peak time. Differences in the other system properties were found to be mainly dependent on the nature of the controller rather than the motif structure. Protein mediated motifs showed a higher degree of adaptation i.e. a tendency to return to baseline levels; in particular, feedforward motifs exhibited perfect adaptation. RNA mediated motifs had a mild regulatory effect; they also exhibited a lower peaking tendency and mean overshoot. Protein mediated feedforward motifs showed higher overshoot and lower peak time compared to the corresponding feedback motifs.1 aIyengar, Bharat, Ravi1 aPillai, Beena1 aVenkatesh, K., V.1 aGadgil, Chetan, J.  uhttps://library.ncl.res.in/content/systematic-comparison-response-properties-protein-and-rna-mediated-gene-regulatory-motifs