01639nas a2200169   4500008004100000245007800041210006900119260000800188300000900196490000700205520109100212100001401303700001401317700001701331700002001348856010101368       2019                        eng d00aMultimodal chromatography for purification of biotherapeutics - a review.0 aMultimodal chromatography for purification of biotherapeutics a   cJAN  a4-130 v203 aProcess chromatography forms the core of purification of biotherapeutics. The unparalleled selectivity that it offers over other alternatives combined with the considerable robustness and scalability make it the unit operation of choice in downstream processing. It is typical to have three to five chromatography steps in a purification process for a biotherapeutic. Generally, these steps offer different modes of separation such as ion-exchange, reversed phase, size exclusion, and hydrophobic interaction. In the past decade, multimodal chromatography has emerged as an alternative to the traditional modes. It involves use of more than one mode of separation and typically combines ion-exchange and hydrophobic interactions to achieve selectivity and sensitivity. Over the last decade, numerous authors have demonstrated the significant potential that multimode chromatography offers as a protein purification tool. This review aims to present key recent developments that have occurred on this topic together with a perspective on future applications of multimodal chromatography.1 aHalan, V.1 aMaity, S.1 aBhambure, R.1 aRathore, A., S.  uhttps://library.ncl.res.in/content/multimodal-chromatography-purification-biotherapeutics-review