01805nas a2200193   4500008004100000022001400041245009300055210007100148260000900219300001600228490000600244520115600250100001401406700001801420700001501438700001701453700001301470856012801483       2018                        eng d  a2470-134300aImpairing powerhouse in colon cancer cells by hydrazide–hydrazone-based small molecule0 aImpairing powerhouse in colon cancer cells by hydrazide–hydrazon  cFEB   a1470–14810 v33 aMitochondrion has emerged as one of the unconventional targets in next-generation cancer therapy. Hence, small molecules targeting mitochondria in cancer cells have immense potential in the next-generation anticancer therapeutics. In this report, we have synthesized a library of hydrazide–hydrazone-based small molecules and identified a novel compound that induces mitochondrial outer membrane permeabilization by inhibiting antiapoptotic B-cell CLL/lymphoma 2 (Bcl-2) family proteins followed by sequestration of proapoptotic cytochrome c. The new small molecule triggered programmed cell death (early and late apoptosis) through cell cycle arrest in the G2/M phase and caspase-9/3 cleavage in HCT-116 colon cancer cells, confirmed by an array of fluorescence confocal microscopy, cell sorting, and immunoblotting analysis. Furthermore, cell viability studies have verified that the small molecule rendered toxicity to a panel of colon cancer cells (HCT-116, DLD-1, and SW-620), keeping healthy L929 fibroblast cells unharmed. The novel small molecule has the potential to form a new understudied class of mitochondria targeting anticancer agent.1 aPatil, S.1 aKuman, M., M.1 aPalvai, S.1 aSengupta, P.1 aBasu, S.  uhttps://library.ncl.res.in/content/impairing-powerhouse-colon-cancer-cells-hydrazide%E2%80%93hydrazone-based-small-molecule