01900nas a2200193   4500008004100000022001400041245011600055210006900171260000800240300001300248490000700261520120700268100001901475700002001494700002301514700001601537700002801553856012501581       2018                        eng d  a1545-788500aHighly diverged novel subunit composition of apicomplexan F-type ATP synthase identified from Toxoplasma gondii0 aHighly diverged novel subunit composition of apicomplexan Ftype   cJUL  ae20061280 v163 a<p>The mitochondrial F-type ATP synthase, a multisubunit nanomotor, is critical for maintaining cellular ATP levels. In T. gondii and other apicomplexan parasites, many subunit components necessary for proper assembly and functioning of this enzyme appear to be missing. Here, we report the identification of 20 novel subunits of T. gondii F-type ATP synthase from mass spectrometry analysis of partially purified monomeric (approximately 600 kDa) and dimeric (&gt; 1 MDa) forms of the enzyme. Despite extreme sequence diversification, key F-O subunits a, b, and d can be identified from conserved structural features. Orthologs for these proteins are restricted to apicomplexan, chromerid, and dinoflagellate species. Interestingly, their absence in ciliates indicates a major diversion, with respect to subunit composition of this enzyme, within the alveolate clade. Discovery of these highly diversified novel components of the apicomplexan F-type ATP synthase complex could facilitate the development of novel antiparasitic agents. Structural and functional characterization of this unusual enzyme complex will advance our fundamental understanding of energy metabolism in apicomplexan species.</p>1 aSalunke, Rahul1 aMourier, Tobias1 aBanerjee, Manidipa1 aPain, Arnab1 aShanmugam, Dhanasekaran  uhttps://library.ncl.res.in/content/highly-diverged-novel-subunit-composition-apicomplexan-f-type-atp-synthase-identified