02829nas a2200241   4500008004100000022001400041245011200055210006900167260000800236300001300244490000700257520196500264100002502229700002402254700002702278700002502305700002602330700002602356700002402382700002902406700002402435856012802459       2018                        eng d  a1935-273500aMetabolite profiling for biomarkers in schistosoma haematobium infection and associated bladder pathologies0 aMetabolite profiling for biomarkers in schistosoma haematobium i  cAPR  ae00064520 v123 a<p>Background Metabolic fingerprinting analysis can offer insights into underlying reactions in a biological system; hence it is crucial to the understanding of disease pathogenesis and could provide useful tools for discovering biomarkers. We sought to examine the urine and plasma metabolome in individuals affected by urogenital schistosomiasis and its associated-bladder pathologies. Methodology Blood and midstream urine were obtained from volunteers who matched our inclusion criteria among residents from Eggua, southwestern Nigeria. Samples were screened by urinalysis, microscopy, PCR and ultrasonography, and categorised as advanced (urogenital schistosomiasis associated-bladder pathologies), infection-only (urogenital schistosomiasis alone) and controls (no infection and no pathology). Metabolites were extracted and data acquired with ultra high-performance liquid chromatography coupled with Thermo Q-Exactive orbitrap HRMS. Data was analysed with MetaboAnalyst, Workflow4Metabolomics, HMDB, LipidMaps and other bioinformatics tools, with univariate and multivariate statistics for metabolite selection. Principal findings There were low levels of host sex steroids, and high levels of several benzenoids, catechols and lipids (including ganglioside, phosphatidylcholine and phosphatidylethanolamine), in infection-only and advanced cases (FDR&lt; 0.05, VIP&gt; 2, delta&gt; 2.0). Metabolites involved in biochemical pathways related to chorismate production were abundant in controls, while those related to choline and sphingolipid metabolism were upregulated in advanced cases FDR&lt;0.05). Some of these human host and Schistosoma haematobium molecules, including catechol estrogens, were good markers to distinguish infection-only and advanced cases. Conclusions Altered glycerophospholipid and sphingolipid metabolism could be key factors promoting the development of bladder pathologies and tumours during urogenital schistosomiasis.</p>1 aAdebayo, Adewale, S.1 aMundhe, Swapnil, D.1 aAwobode, Henrietta, O.1 aOnile, Olugbenga, S.1 aAgunloye, Atinuke, M.1 aIsokpehi, Raphael, D.1 aShouche, Yogesh, S.1 aSanthakumari, Bayatigeri1 aAnumudu, Chiaka, I.  uhttps://library.ncl.res.in/content/metabolite-profiling-biomarkers-schistosoma-haematobium-infection-and-associated-bladder