01531nas a2200145   4500008004100000245005500041210005400096260000800150300001200158490000900170520107500179100002101254700002001275856009001295       2018                        eng d00aIntegrated host-pathogen metabolic reconstructions0 aIntegrated hostpathogen metabolic reconstructions  cJAN  a197-2170 v17163 aThe science and art of Genome scale metabolic network reconstructions have been explicitly documented in the literature for organisms across all the three kingdoms of life. Constraints-based models derived from such reconstructions have been used to assess metabolic phenotypes of their complex connections to genotype accurately. The problem of infectious disease is complex due to the multifactorial response of the host to the pathogen. Systems biology approaches and modeling allow one to study, understand, and predict emergent properties of such complex responses. The integration of the host and pathogen metabolic networks and the subsequent merger of their stoichiometric matrices is nontrivial and requires understanding of both pathogen and host metabolism and physiologies. The protocol here describes the detailed process of network and stoichiometric matrix merger using a salmonella-mouse macrophage model. The protocol also discusses the interfacial and objective functions required to actually embark on the analysis of host-pathogen interaction models.1 aRaghunathan, Anu1 aJamshidi, Neema  uhttps://library.ncl.res.in/content/integrated-host-pathogen-metabolic-reconstructions