02082nas a2200217   4500008004100000245013100041210006900172260000800241300002500249490000600274520129300280100001701573700001901590700002001609700001801629700002101647700002301668700001801691700002601709856012901735       2018                        eng d00aSilk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration0 aSilk fibroin microparticle scaffolds with superior compression m  cMAY  aArticle Number: 72350 v83 aSilk fibroin (SF), a natural polymer produced by Bombyx mori silkworms, has been extensively explored to prepare porous scaffolds for tissue engineering applications. Here, we demonstrate, a scaffold made of SF, which exhibits compression modulus comparable to natural cancellous bone while retaining the appropriate porosities and interconnected pore architecture. The scaffolds also exhibit high resistance to in-vitro proteolytic degradation due to the dominant beta sheet conformation of the SF protein. Additionally, the scaffolds are prepared using a simple method of microparticle aggregation. We also demonstrate, for the first time, a method to prepare SF micro-particles using a Hexafluoroisopropanol-Methanol solvent-coagulant combination. SF microparticles obtained using this method are monodisperse, spherical, non-porous and extremely crystalline. These micro-particles have been further aggregated together to form a 3D scaffold. The aggregation is achieved by random packing of these microparticles and fusing them together using a dilute SF solution. Preliminary in-vitro cell culture and in-vivo implantation studies demonstrate that the scaffolds are biocompatible and they exhibit the appropriate early markers, making them promising candidates for bone regeneration.1 aNisal, Anuya1 aSayyad, Raeesa1 aDhavale, Prachi1 aKhude, Bhakti1 aDeshpande, Rucha1 aMapare, Vidhyashri1 aShukla, Swati1 aVenugopalan, Premnath  uhttps://library.ncl.res.in/content/silk-fibroin-micro-particle-scaffolds-superior-compression-modulus-and-slow-bioresorption