02498nas a2200265   4500008004100000245016500041210006900206260000800275300001500283490000600298520155600304100002101860700001801881700002001899700001701919700002301936700002101959700001801980700002401998700002202022700001402044700002302058700002202081856012902103       2017                        eng d00aSelective isolation and eradication of E. coli associated with urinary tract infections using anti-fimbrial modified magnetic reduced graphene oxide nanoheaters0 aSelective isolation and eradication of E coli associated with ur  cOCT  a 8133-81420 v53 aThe fast and efficient elimination of pathogenic bacteria from water, food or biological samples such as blood remains a challenging task. Magnetic isolation of bacteria from complex media holds particular promise for water disinfection and other biotechnological applications employing bacteria. When it comes to infectious diseases such as urinary tract infections, the selective removal of the pathogenic species in complex media such as human serum is also of importance. This issue can only be accomplished by adding pathogen specific targeting sites onto the magnetic nanostructures. In this work, we investigate the potential of 2-nitrodopamine modified magnetic particles anchored on reduced graphene oxide (rGO) nanocomposites for rapid capture and efficient elimination of E. coli associated with urinary tract infections (UTIs) from water and serum samples. An optimized magnetic nanocarrier achieves a 99.9% capture efficiency even at E. coli concentrations of 1 x 10(1) cfu mL(-1) in 30 min. In addition, functionalization of the nanostructures with poly(ethylene glycol) modified pyrene units and anti-fimbrial E. coli antibodies allowed specific elimination of E. coli UTI89 from serum samples. Irradiation of the E. coli loaded nanocomposite with a near-infrared laser results in the total ablation of the captured pathogens. This method can be flexibly modified for any other pathogenic bacteria, depending on the antibodies used, and might be an interesting alternative material for a magnetic-based body fluid purification approach.1 aHalouane, Fatima1 aJijie, Roxana1 aMeziane, Dalila1 aLi, Chengnan1 aSingh, Santosh, K.1 aBouckaert, Julie1 aJurazek, Jean1 aKurungot, Sreekumar1 aBarras, Alexandre1 aLi, Musen1 aBoukherroub, Rabah1 aSzunerits, Sabine  uhttps://library.ncl.res.in/content/selective-isolation-and-eradication-e-coli-associated-urinary-tract-infections-using-anti