02432nas a2200193   4500008004100000245014200041210006900183260000800252300000900260520172500269653001401994653001602008100001502024700002202039700001802061700001802079700001502097856012602112       2017                        eng d00aViable alternative in vitro system and comparative metabolite profiling of different tissues for the conservation of ceropegia karulensis0 aViable alternative in vitro system and comparative metabolite pr  cAUG  a1-153 aAbstract: Ceropegia karulensis is an endemic and critically endangered plant of the Western Ghats from India. Exploitation of the tubers and poor regeneration from seed has narrowed distribution and propagation of the species. There is a need to develop in vitro propagation methods for C. karulensis to alleviate these problems. Here, we optimized callus induction, somatic embryogenesis and microtuberization from different seedling explants viz. cotyledonary leaf and root. The environmental scanning electron microscopy was used to observe somatic embryonic origin and their developmental stages. Highest callus proliferation was recorded with 2 µM 6-benzylaminopurine and 1 µM 2,4-dichlorophenoxyacetic acid. Somatic embryos derived from cotyledonary leaf explants were more proliferative than root explants. The combination of 2 µM 6-benzylaminopurine, 2 µM naphthalene acetic acid and 7% sucrose in MS media resulted in highest microtuberization. Further, gas chromatography-mass spectrometry based metabolic profiling was carried out from native wild plants and in vitro callus tissues which identified various phytochemicals such as alkaloids, fatty acids, esters alcohols, etc. Multivariate analysis revealed the chemical disparities, where considerable variations were observed between native wild type and in vitro tissues, but no significant differences were found among in vitro callus from both root and cotyledonary explants. Overall, our results suggested that the production of various secondary metabolites found in C. karulensis was not affected by in vitro propagation and could be utilized in the conservation strategies for this plant. Graphical Abstract: [Figure not available: see fulltext.]10aalkaloids10aMetabolites1 aPandey, M.1 aJayaramaiah, R.H.1 aDholakia, B.B1 aPunekar, S.A.1 aGiri, A.P.  uhttps://library.ncl.res.in/content/viable-alternative-vitro-system-and-comparative-metabolite-profiling-different-tissues