01462nas a2200145   4500008004100000245008700041210006900128300001000197490000900207520091800216100002301134700002501157700002201182856011201204       2017                        eng d00aMolecular methods for identification of clostridium tetani by targeting neurotoxin0 aMolecular methods for identification of clostridium tetani by ta  a37-470 v16003 aTetanus is a potentially fatal muscle spasm disease. It is an important public health problem, especially in rural/tribal areas of developing countries. Tetanus toxin, a neurotoxin (tetanospasmin ), is the most important virulence factor that plays a key role in the pathogenicity of tetanus . Confirmation of virulence by confirming the production of tetanospasmin by infecting species forms the most important part in the diagnosis of tetanus . Various molecular methods have been devised for confirmation of diagnosis by targeting different genes. The most common molecular methods are tetanospasmin producing (TetX) gene-targeted methods using TetX-specific primers. Here, we describe various molecular methods targeting TetX gene such as polymerase chain reaction, pulsed-field gel electrophoresis, Southern blotting, loop-mediated isothermal amplification assay, etc. to confirm the virulence of Cl. tetani.1 aDharne, Mahesh, S.1 aNagoba, Basavraj, S.1 aGohil, Kushal, N.  uhttps://library.ncl.res.in/content/molecular-methods-identification-clostridium-tetani-targeting-neurotoxin