02201nas a2200193   4500008004100000245010800041210006900149260000800218300001200226490000700238520154100245100001601786700001501802700001601817700002201833700001301855700001501868856012401883       2017                        eng d00aData on the effect of oral feeding of Arachidonic acid or Docosahexanoic acid on haematopoiesis in mice0 aData on the effect of oral feeding of Arachidonic acid or Docosa  cOCT  a551-5570 v143 a<p>Stem cells have peculiar property to self-renew and differentiate. It is important to control their fate in safe and effective ways for their therapeutic use. The mediators of essential polyunsaturated fatty acids (PUFAs) namely Arachidonic acid (AA) and Docosahexanoic acid (DHA) are known to play a role in haematopoiesis via various metabolic pathways [1]. However the direct effect of purified AA or DHA on haematopoiesis has not been well investigated yet. We have reported that oral administration of PUFAs enhanced haematopoiesis in mice [2]. Signaling Leukocyte Antigen Molecule (SLAM) (CD48−CD150+) phenotype consists of pure population of haematopoietic stem cells (HSCs). Herein we observed higher percentage of SLAM (CD48−CD150+) phenotype in the bone marrow (BM) cells of mice fed with AA or DHA compared to PBS fed control mice. Data from engraftment study depicts that BM from AA/DHA-fed mice showed higher absolute number of donor cells in recipient mice compared to control. The enhanced hematopoiesis observed in AA/DHA-fed mice was returned to normal when the mice were kept on normal diet for six weeks (after ten days of oral feeding). We confirmed GCMS (Gas Chromatography-Mass Spectroscopy) retention times of AA and DHA by co-injecting fatty acid extract from AA or DHA fed mice with purified AA or DHA standards respectively. Representative flow cytometry profile of Lin−Sca-1+c-kit+(LSK) cells showed higher expression of CXCR4 protein and ligands of Wnt, Notch1 signaling in BM of AA/DHA-fed mice</p>1 aLimbkar, K.1 aDhenge, A.1 aJadhav, D.D1 aThulasiram, H. V.1 aKale, V.1 aLimaye, L.  uhttps://library.ncl.res.in/content/data-effect-oral-feeding-arachidonic-acid-or-docosahexanoic-acid-haematopoiesis-mice