01775nas a2200241   4500008004100000245010900041210006900150260000800219300001600227490000600243520093200249100002701181700002201208700001901230700002601249700002501275700001701300700002601317700002001343700003201363700001601395856012201411       2017                        eng d00aAnti-microbial activity of chrysomycin a produced by streptomyces sp. against mycobacterium tuberculosis0 aAntimicrobial activity of chrysomycin a produced by streptomyces  cJUL  a36335-363390 v73 aLimited efficacy of the BCG (Bacillus Calmette–Guérin) vaccine against adult tuberculosis and the emergence of resistance to existing anti-tuberculosis drugs compel discovery of novel antibiotics against Mycobacterium tuberculosis. Actinomycetes are still an attractive platform for the discovery of new antimicrobials, especially from untapped natural hotspots, despite the belief that they are an exhausted resource after repeated re-discoveries. Herein we report the isolation and identification of chrysomycin A from an actinomycete isolated from a coastal area in Kerala. We show for the first time that it has antimycobacterial activity. It was found to be bactericidal to planktonic and intracellular M. tuberculosis with an MIC of 3.125 μg mL⁻¹; it is non-hemolytic and has negligible cytotoxicity. The actinomycete that produces chrysomycin A was found to be a Streptomyces sp. through 16S rRNA gene sequencing.1 aBalaji, Muralikrishnan1 aDan, Vipin, Mohan1 aJoseph, Vinodh1 aJamsheena, Vellekkatt1 aRamachandran, Ranjit1 aThomas, Sabu1 aDastager, Syed, Gulam1 aKumar, Santhosh1 aLankalapalli, Ravi, Shankar1 aKumar, Ajay  uhttps://library.ncl.res.in/content/anti-microbial-activity-chrysomycin-produced-streptomyces-sp-against-mycobacterium