03063nas a2200361   4500008004100000245013600041210006900177260000800246490000700254520182400261653003802085653001502123653002002138653002202158653001802180653003602198653001602234653002602250653003202276653002102308653002102329653001702350653002002367653002802387653001902415100001902434700001902453700002302472700003702495700002102532700001902553856012902572       2017                        eng d00aOral feeding with polyunsaturated fatty acids fosters hematopoiesis and thrombopoiesis in healthy and bone marrow-transplanted mice0 aOral feeding with polyunsaturated fatty acids fosters hematopoie  csep0 v473 a<p><span style="color: rgb(51, 51, 51); font-family: arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(248, 248, 248);">Hematopoietic stem cells play the vital role of maintaining appropriate levels of cells in blood. Therefore, regulation of their fate is essential for their effective therapeutic use. Here we report the role of polyunsaturated fatty acids (PUFAs) in regulating hematopoiesis which has not been explored well so far. Mice were fed daily for 10 days with n-6/n-3 PUFAs, viz. linoleic acid (LA), arachidonic acid (AA), alpha-linolenic acid and docosahexanoic acid (DHA) in four separate test groups with phosphate-buffered saline fed mice as control set. The bone marrow cells of PUFA-fed mice showed a significantly higher hematopoiesis as assessed using side population, Lin-Sca-1(+)ckit+, colony-forming unit (CFU), long-term culture, CFU-spleen assay and engraftment potential as compared to the control set. Thrombopoiesis was also stimulated in PUFA-fed mice. A combination of DHA and AA was found to be more effective than when either was fed individually. Higher incorporation of PUFAs as well as products of their metabolism was observed in the bone marrow cells of PUFA-fed mice. A stimulation of the Wnt, CXCR4 and Notch1 pathways was observed in PUFA-fed mice. The clinical relevance of this study was evident when bone marrow-transplanted recipient mice, which were fed with PUFAs, showed higher engraftment of donor cells, suggesting that the bone marrow microenvironment may also be stimulated by feeding with PUFAs. These data indicate that oral administration of PUFAs in mice stimulates hematopoiesis and thrombopoiesis and could serve as a valuable supplemental therapy in situations of hematopoietic failure. (C) 2017 Elsevier Inc. All rights reserved.</span></p>10aBone Marrow Transplantation (Bmt)10aCord Blood10aDown-regulation10aEx-vivo Expansion10aHematopoiesis10aHematopoietic Stem Cells (Hscs)10aLipid rafts10aMyocardial-infarction10aPolyunsaturated Fatty Acids10aProgenitor Cells10aProstaglandin-e210aSelf-renewal10aSpleen Colonies10aStem-cell Proliferation10aThrombopoiesis1 aLimbkar, Kedar1 aDhenge, Ankita1 aJadhav, Dipesh, D.1 aThulasiram, Hirekodathakallu, V.1 aKale, Vaijayanti1 aLimaye, Lalita  uhttps://library.ncl.res.in/content/oral-feeding-polyunsaturated-fatty-acids-fosters-hematopoiesis-and-thrombopoiesis-healthy