01948nas a2200217   4500008004100000245014600041210006900187260000800256300001400264490000700278520115500285100001801440700002101458700002001479700002701499700002301526700001501549700002101564700002101585856012401606       2017                        eng d00aGlucosylation of smoke-derived volatiles in grapevine (vitis vinifera) is catalyzed by a promiscuous resveratrol/guaiacol glucosyltransferase0 aGlucosylation of smokederived volatiles in grapevine vitis vinif  cJUL  a5681-56890 v653 aVinification of grapes (Vitis vinifera) exposed to forest fire smoke can yield unpalatable wine due to the presence of taint compounds from smoke and the release of smoke derived volatiles from their respective glycosides during the fermentation process or in-mouth during consumption. To identify glycosyltransferases (GTs) involved in the formation of glycosidically bound smoke-derived volatiles we performed gene expression analysis of candidate GTs in different grapevine tissues. Second, substrates derived from bushfire smoke or naturally occurring in grapes were screened with the candidate recombinant GTs. A resveratrol GT (UGT72B27) gene, highly expressed in grapevine leaves and berries was identified to be responsible for the production of the phenolic glucosides. UGT72B27 converted the stilbene trans-resveratrol mainly to the 3-O-glucoside. Kinetic analyses yielded specificity constants (k(cat)/K-m) of 114, 17, 9, 8, and 2 mM(-1) s(-1) for guaiacol, trans-resveratrol, syringol, methylsyringol, and methylguaiacol, respectively. This knowledge will help to design strategies for managing the risk of producing smoke-affected wines.1 aHaratl, Katja1 aHuang, Fong-Chin1 aGiri, Ashok, P.1 aFranz-Oberdorf, Katrin1 aFrotscher, Johanna1 aShao, Yang1 aHoffmann, Thomas1 aSchwab, Wilfried  uhttps://library.ncl.res.in/content/glucosylation-smoke-derived-volatiles-grapevine-vitis-vinifera-catalyzed-promiscuous