02263nas a2200169   4500008004100000245015100041210006900192260000800261300001400269490000600283520159700289653002101886653001501907100002701922700001701949856012701966       2017                        eng d00api-Conjugated chromophore incorporated polystyrene nanobeads as single optical agent for three-channel fluorescent probe in bioimaging application0 apiConjugated chromophore incorporated polystyrene nanobeads as s  cAUG  a1788-17980 v33 a<p>Fluorescent polystyrene (PS) nanobeads in the size range similar to 70-120 nm incorporating perylene bisimide (PBI-PS) and/or oligo(p-phenylenevinylene) (OPV-PS) was developed by miniemulsion polymerization technique. A dye loading content (DLC) of &lt;3% was sufficient to impart high fluorescence emission capability to the PS beads. OPV-PS exhibited emission in the range 400-550 nm with peak emission at 450 nm (lambda(ex) = 350 nm; phi(FL) = 26%); PBI-PS showed emission from 520-650 nm with peak emission at 545 nm (lambda(ex) = 490 nm; phi(FL) = 9.7%) in 1x PBS buffer, whereas OPV(PBI)-PS nanobeads incorporating both the fluorophores exhibited multicolor emission capabilities (lambda(ex) from 350 to 490 nm). The nanoparticles were characterized by field-emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and dynamic light scattering (DLS) for size and zeta potential for surface charge. For bioimaging applications, the PS nanoparticles were incubated with HeLa cells. Cell viability analysis involving HeLa cells showed more than 90% cell viability confirming the biocompatibility of the PS The cellular uptake of the nanoparticles was confirmed by flow cytometry analysis and confocal laser scanning microscopy (CLSM) images. The subcellular localization of the nanoparticles in the cytoplasm could be precisely established by their simultaneous multicolor emission. The PS-based single optical agent presented here that can function as three-channel fluorescent probe to meet the requirements for multicolor bioimaging is advantageous.</p>10aBiocompatibility10abioimaging1 aMakkad, Sarabjot, Kaur1 aAsha, S., K.  uhttps://library.ncl.res.in/content/pi-conjugated-chromophore-incorporated-polystyrene-nanobeads-single-optical-agent-three