01720nas a2200217   4500008004100000245009800041210006900139260000800208300001400216490000700230520096100237100001801198700002201216700002101238700002901259700001901288700002301307700002101330700002401351856012701375       2017                        eng d00aAcetone and methanol fruit extracts of terminalia paniculata inhibit HIV-1 infection in vitro0 aAcetone and methanol fruit extracts of terminalia paniculata inh  cJAN  a1468-14710 v313 aIn this study, we report the in vitro anti-HIV1 activity of acetone and methanol extracts of fruit of Terminalia paniculata. Cytotoxicity tests were conducted on TZM-bl cells and peripheral blood mononuclear cells (PBMC), the CC50 values of both the extracts were 260g/mL. Using TZM-bl cells, the extracts were tested for their ability to inhibit replication of two primary isolates HIV-1 (X4, Subtype D) and HIV-1 (R5, Subtype C). The activity against HIV-1 primary isolate (R5, Subtype C) was confirmed using activated PBMC and by quantification of HIV-1 p24 antigen. Both the extracts showed anti-HIV1 activity in a dose-dependent manner. The EC50 values of the acetone and methanol extracts of T. paniculata were 10.3g/mL. The enzymatic assays were performed to determine the mechanism of action which indicated that the anti-HIV1 activity might be due to inhibition of reverse transcriptase (77.7% inhibition) and protease (69.9% inhibition) enzymes. 1 aDurge, Ankita1 aJadaun, Pratiksha1 aWadhwani, Ashish1 aChinchansure, Ashish, A.1 aSaid, Madhukar1 aThulasiram, H., V.1 aJoshi, Swati, P.1 aKulkarni, Smita, S.  uhttps://library.ncl.res.in/content/acetone-and-methanol-fruit-extracts-terminalia-paniculata-inhibit-hiv-1-infection-vitro