02221nas a2200277   4500008004100000245013800041210006900179260000800248300002600256490000600282520122300288100002201511700002701533700003301560700002301593700001701616700001801633700002301651700001901674700002701693700002101720700002301741700002701764700002101791856013101812       2016                        eng d00aEvaluation of uttroside B, a saponin from Solanum nigrum Linn, as a promising chemotherapeutic agent against hepatocellular carcinoma0 aEvaluation of uttroside B a saponin from Solanum nigrum Linn as   cNOV  aArticle Number: 363180 v63 aWe report, for the first time, the remarkable efficacy of uttroside B, a potent saponin from Solanum nigrum Linn, against liver cancer. The compound has been isolated and characterized from the leaves of Solanum nigrum Linn, a plant widely used in traditional medicine and is a rich resource of several anticancer molecules. Uttroside B, that comprises of beta-D-glucopyranosyl unit at C-26 of the furostanol and beta-lycotetraosyl unit at C-3, is ten times more cytotoxic to the liver cancer cell line, HepG2 (IC50: 0.5 mu M) than sorafenib (IC50: 5.8 mu M), the only FDA-approved drug for liver cancer. Moreover, it induces cytotoxicity in all liver cancer cell lines, irrespective of their HBV status, while being non-toxic to normal immortalized hepatocytes. It induces apoptosis in HepG2 cells by down-regulating mainly the activation of MAPK and mTOR pathways. The drastic reduction in HepG2-xenograft tumor size achieved by uttroside B in NOD-SCID mice and substantiation of its biological safety through both acute and chronic toxicity studies in Swiss albino mice warrants clinical validation of the molecule against hepatic cancer, for which, the chemotherapeutic armamentarium currently has limited weapons.1 aNath, Lekshmi, R.1 aGorantla, Jaggaiah, N.1 aThulasidasan, Arun, Kumar T.1 aVijayakurup, Vinod1 aShah, Shabna1 aAnwer, Shabna1 aJoseph, Sophia, M.1 aAntony, Jayesh1 aVeena, Kollery, Suresh1 aSundaram, Sankar1 aMarelli, Udaya, K.1 aLankalapalli, Ravi, S.1 aAnto, Ruby, John  uhttps://library.ncl.res.in/content/evaluation-uttroside-b-saponin-solanum-nigrum-linn-promising-chemotherapeutic-agent-against