01572nas a2200169   4500008004100000245008700041210006900128260000800197300002400205490000600229520096500235100002101200700002501221700002501246700001901271856011201290       2017                        eng d00aSuperoxide generation and its involvement in the growth of mycobacterium smegmatis0 aSuperoxide generation and its involvement in the growth of mycob  cJAN  aArticle Number: 1050 v83 a<p>Superoxide generation is inevitable in aerobic organisms, most of which have developed mechanisms to detoxify superoxides. However, its significance has not been clearly understood in mycobacteria. This study demonstrates that NADH oxidase is the major source of superoxide in Mycobacterium smegmatis and elucidates the involvement of superoxide in M. smegmatis growth. The maximum inhibition of superoxide generation was observed in the presence of diphenyleneiodonium chloride (DPI), an NADH oxidase inhibitor, compared to other standard inhibitors. After incubation for 24 h, the number of colony forming units (CFUs) was reduced by 6.8 log10 compared to the untreated culture. The inhibitory effect of DPI on M. smegmatis was reversed when the same culture was supplemented with menadione and pyrogallol, which are superoxide generators. Thus, this study reports the source of superoxide generation and its involvement in the growth of M. smegmatis.</p>1 aYeware, Amar, M.1 aShurpali, Ketaki, D.1 aAthalye, Meghana, C.1 aSarkar, Dhiman  uhttps://library.ncl.res.in/content/superoxide-generation-and-its-involvement-growth-mycobacterium-smegmatis