01479nas a2200121   4500008004100000245010000041210006900141260009100210520088800301100001901189700001901208856013001227       2015                        eng d00aQuantitative, automated, high content screening assay approach to anti-angiogenicdrug discovery0 aQuantitative automated high content screening assay approach to   aCombi Chem-Bio Resource Center, OCD, National Chemical Laboratory, Pune - 411 008cFEB3 a<p>Angiogenesisenhancescancer,maculardegeneration,etc.. Currentangiogenicassays--indirect,manual,timeconsuming,laborintensive,difficulttoquantify,andexpensive. Wedevelopedanautomated,quantitative,HighContentScreeningangiogenictubeformationassaywouldbenefitdrugdiscoveryforseveraltherapeuticareas. Humanendothelialcellswereseededwithpro-oranti-angiogeniccompoundsonaMatrigelTMlayerina96-wellplate.Afterangiogenictubesformation,thepositivecontrolsandthetestsampleswerefixed,fluorescentlylabeled,automaticallyimagedonCellomics’ArrayScan®HCSReader,andquantitativelyanalyzedon-the-flybytheTubeFormationBioApplicationsoftwaremodule. Multiplefeaturesrelevantforangiogenesisandangiogenictubeformationwereautomaticallymeasured. Thisrobustandreliableassaythusenabledthelarge-scale,quantitative,screeningandcharacterizationofcompoundsaffectingbothpro-andanti-angiogenicpathways.</p>1 aNawale, Laxman1 aSarkar, Dhiman  uhttps://library.ncl.res.in/content/quantitative-automated-high-content-screening-assay-approach-anti-angiogenicdrug-discovery