02563nas a2200229   4500008004100000245012400041210006900165260000800234300001400242490000700256520177300263100002602036700001702062700001702079700001502096700001702111700001902128700001702147700002402164700001702188856012802205       2016                        eng d00aBiosynthesis of anti-proliferative gold using endophytic fusarium oxysporum strain isolated from neem (A-indica) leaves0 aBiosynthesis of antiproliferative gold using endophytic fusarium  cJAN  a2036-20420 v163 a<p>Here we report a simple, rapid, environment friendly approach for the synthesis of gold nanoparticles using neem (Azadirachta indica A. Juss.) fungal endophyte, which based upon morphological and cultural characteristics was eventually identified as Fusarium oxysporum. The aqueous precursor (HAuCl4) solution when reacted with endophytic fungus resulted in the biosynthesis of abundant amounts of well dispersed gold nanoparticles of 10-40 nm with an average size of 22nm. These biosynthesized gold nanoparticles were then characterized by standard analytical techniques such as UV-Visible spectroscopy, X-ray diffraction, Transmission Electron Microscopy and Fourier Transform Infrared Spectroscopy. Cytotoxic activity of these nanoparticles was checked against three different cell types including breast cancer (ZR-75-1), Daudi (Human Burkitt's lymphoma cancer) and normal human peripheral blood mononuclear cells (PBMC), where it was found that our gold nanoparticles are anti-proliferative against cancer cells but completely safe toward normal cells. In addition to this, assessment of toxicity toward human RBC revealed less than 0.1 % hemolysis as compared to Triton X-100 suggesting safe nature of our biosynthesized gold nanoparticles on human cells. Also, our nanoparticles exhibited no anti-fungal (against Aspergillus niger) or anti-bacterial [against Gram positive (Bacillus subtilis &amp; Staphylococcus aureus) and Gram negative (Escherichia coli &amp; Pseudomonas aeruginosa) bacteria] activity thus suggesting their non-toxic, biocompatible nature. The present investigation opens up avenues for eco-friendly, biocompatible nanomaterials to be used in a wide variety of application such as drug delivery, therapeutics, theranostics and so on.</p>1 aSiddiqui, Ejaz, Ahmad1 aAhmad, Absar1 aJulius, Anju1 aSyed, Asad1 aKhan, Shadab1 aKharat, Mahesh1 aPai, Kalpana1 aKadoo, Narendra, Y.1 aGupta, Vidya  uhttps://library.ncl.res.in/content/biosynthesis-anti-proliferative-gold-using-endophytic-fusarium-oxysporum-strain-isolated