02159nas a2200229   4500008004100000022001400041245016500055210006900220260008600289300001200375490000700387520123900394653001001633653001801643653001601661653002501677100002901702700002401731700002301755700002101778856013001799       2016                        eng d  a1082-606800aPurification and partial characterization of novel penicillin V acylase from Acinetobacter sp AP24 isolated from loktak lake, an indo-burma biodiversity hotspot0 aPurification and partial characterization of novel penicillin V   a530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USAbTAYLOR & FRANCIS INCcOCT  a524-5300 v463 a<p>Members of the bacterial genus Acinetobacter have attracted great attention over the past few decades, on account of their various biotechnological applications and clinical implications. In this study, we are reporting the first experimental penicillin V acylase (PVA) activity from this genus. Penicillin acylases are pharmaceutically important enzymes widely used in the synthesis of semisynthetic beta-lactam antibiotics. The bacterium, identified as Acinetobacter sp. AP24, was isolated from the water of Loktak Lake (Manipur, India), an Indo-Burma biodiversity hotspot. PVA production was increased threefold in an optimized medium with 0.2% sodium glutamate and 1% glucose as nitrogen and carbon sources respectively, after 24hr of fermentation at 28 degrees C and pH 7.0 with shaking at 180rpm. The enzyme was purified to homogeneity by cation-exchange chromatography using SP-sepharose resin. The PVA is a homotetramer with subunit molecular mass of 34 kD. The enzyme was highly specific toward penicillin V with optimal hydrolytic activity at 40 degrees C and pH 7.5. The enzyme was stable from pH 5.0 to 9.0 at 25 degrees C for 2hr. The enzyme retained 75% activity after 1hr of incubation at 40 degrees C at pH 7.5.</p>10a6-APA10aAcinetobacter10aLoktak Lake10aPenicillin V acylase1 aPhilem, Pushparani, Devi1 aSonalkar, Vidya, V.1 aDharne, Mahesh, S.1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/purification-and-partial-characterization-novel-penicillin-v-acylase-acinetobacter-sp-ap24