02180nas a2200253   4500008004100000022001400041245009300055210006900148260009500217300001000312490000700322520129400329653001601623653002101639653001901660653001401679653002201693653001201715100001901727700002301746700001801769700002401787856011501811       2016                        eng d  a0960-308500aImmobilization of pectinase on reusable polymer support for clarification of apple juice0 aImmobilization of pectinase on reusable polymer support for clar  a165-189 RAILWAY TERRACE, DAVIS BLDG, RUGBY CV21 3HQ, ENGLANDbINST CHEMICAL ENGINEERScJUL  a12-190 v993 a<p>Pectinase (E.C.3.2.1.15) was successfully immobilized on recyclable polymer matrix. The immobilization matrix was prepared by reaction of polyethyleneimine (mol. wt. 70,000) with epoxy-activated acrylate copolymer DILBEAD-VWR. The enzyme pectinase was first adsorbed on the polymer at pH 7.0 via ion exchange and then stabilized by crosslinking with dextran aldehyde. While the free enzyme shows a pH-optimum of 5.0, the immobilized enzyme exhibited high level of activity in a broad pH range of pH 3.0-7.0. Although the thermal stability of free and immobilized enzymes was similar, at room temperature, the immobilized enzyme could be recycled more than 10 times with loss of less than 5% of its activity during clarification of apple juice. On the eventual loss of enzyme activity, the immobilized enzyme and dextran aldehyde can be easily removed from the polymer by a simple treatment with 1N HCl and the polymer can be reused for immobilization of a fresh batch of enzyme. This support also can be reused several times, making the process economically attractive. The properties of apple juice treated with immobilized enzyme were similar to those of that treated with free pectinase. (C) 2016 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.</p>10aApple juice10aDextran aldehyde10aImmobilization10aPectinase10aPolyethyleneimine10aPolymer1 aRajdeo, Kishor1 aHarini, Tirunagari1 aLavanya, Kuna1 aFadnavis, Nitin, W.  uhttps://library.ncl.res.in/content/immobilization-pectinase-reusable-polymer-support-clarification-apple-juice