02072nas a2200265   4500008004100000022001400041245011400055210006900169260011700238300001000355490000600365520106900371653002801440653001501468653003701483653001701520653001401537653001101551100003101562700002401593700002001617700002201637700002301659856012401682       2007                        eng d  a0973-626300aImmobilization of invertase on functionalized mesoporous silica and properties of the immobilized biocatalyst0 aImmobilization of invertase on functionalized mesoporous silica   aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcFEB  a12-170 v23 a<p>Invertase, which catalyses the hydrolysis of sucrose, is one of the simplest commercial carbohydrases and is used for the production of invert syrup (mixture of glucose and fructose). Fructose is the sweetest natural sugar having beneficial medicinal effects and is emerging as an alternative sweetener to sucrose in the pharmaceutical industry. beta fructofuranosidase (E C 3.2.1.26) purified from Kluyveromyces marxianus NCYC 2675, a thermotolerant yeast, was immobilized on functionalized mesoporous silica SBA- 15. Attempts were made to immobilize invertase on functionalized silicalite and cross linked with glutaraldehyde so as to obtain a highly active and stable immobilized enzyme preparation suitable for commercial exploitation. Cross linked enzyme silica complex as well as enzyme silica complex showed very good temperature and pH stability. Extrudates of the mesoporous silica also showed similar results. Bound enzyme showed excellent stability to repeated use and retained 90% of its initial activity after ten cycles of reuse in batch mode.</p>10abeta fructofuranosidase10aextrudates10afunctionalized mesoporous silica10aInvert syrup10ainvertase10aSBA-151 aPrabhune, Asmita, Ashutosh1 aSulabha, Karandikar1 aArchana, Pundle1 aShilpa, Deshpande1 aSanjeevani, Pardhy  uhttps://library.ncl.res.in/content/immobilization-invertase-functionalized-mesoporous-silica-and-properties-immobilized