02016nas a2200229   4500008004100000022001400041245009600055210006900151260009900220300001400319490000700333520110200340653004001442653002301482653003301505653002901538100002201567700003001589700002301619700002001642856012401662       2007                        eng d  a0960-852400aStrain improvement of penicillium janthinellum ncim 1171 for increased cellulase production0 aStrain improvement of penicillium janthinellum ncim 1171 for inc  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcMAY  a1467-14730 v983 a<p>The strain of Penicilhuinjanthinellunt NOM 1171 was subjected to mutation involving treatment of Ethyl Methyl Sulfonate (EMS) for 24 h followed by UV-irradiation for 3 min. Successive mutants showed enhanced cellulase production (EMS-UV-8), clearance zone on Avicel containing plate (SM2) and rapid growth on Walseth cellulose agar plates containing 0.2% 2-deoxy-D-glucose (SM3). These mutants were transferred to Walseth cellulose plates containing higher concentration (1.5%) of 2-deoxy-D-glucose (SM4) in which only five mutants showed clearance zone on SM4. All these mutants showed approximately two-fold increase in activity of both FPase and CMCase in shake flask culture when grown on basal medium containing CP-123 (1%) and wheat bran (2.5%). The enzyme preparations from these mutants were used to hydrolyze Avicel. Higher hydrolysis yields of Avicel were obtained with enzyme preparations of EU1. This is the first report on the isolation and selection of mutants based on hydrolysis of Avicel, which is the most crystalline substrate. (c) 2006 Elsevier Ltd. All rights reserved.</p>10a2-deoxy-D-glucose resistant mutants10acellulase activity10aEMS and ultraviolet mutation10aPenicillium janthinellum1 aAdsul, Mukund, G.1 aBastawade, Kulbhushan, B.1 aVarma, Anjanikumar1 aGokhale, D., V.  uhttps://library.ncl.res.in/content/strain-improvement-penicillium-janthinellum-ncim-1171-increased-cellulase-production