01738nas a2200169   4500008004100000022001400041245009500055210006900150260008000219300001400299490000700313520106700320100001901387700002001406700001801426856012401444       2007                        eng d  a0022-372700aPoly(o-anisidine) films on mild steel: electrochemical synthesis and biosensor application0 aPolyoanisidine films on mild steel electrochemical synthesis and  aDIRAC HOUSE, TEMPLE BACK, BRISTOL BS1 6BE, ENGLANDbIOP PUBLISHING LTDcAPR  a2555-25620 v403 a<p>Poly(o-anisidine) (POA) films were synthesized on mild steel from an aqueous oxalic acid solution by electrochemical polymerization of o-anisidine using cyclic voltammetry. These films were characterized by cyclic voltammetry, UV-visible absorption spectroscopy, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The enzyme glucose oxidase (GOx) was entrapped into the POA film by a physical adsorption method. The resulting POA-GOx films were characterized by UV-visible absorption spectroscopy, FTIR and SEM. The amperometric response of the POA-GOx films was measured as a function of glucose concentration in phosphate buffer solution (pH 7.3). The POA-GOx films exhibited a fast amperometric response (1-5 s) and a linear response in the range of 2-20 mM glucose. The maximum current density and Michaelis-Menten constant of POA/GOx films were found to be similar to 406 mu A cm(-2) and 1.03 mM, respectively. The shelf stability, operational stability and thermal stability of these films were also investigated.</p>1 aPatil, Dewyani1 aGaikwad, A., B.1 aPatil, Pradip  uhttps://library.ncl.res.in/content/polyo-anisidine-films-mild-steel-electrochemical-synthesis-and-biosensor-application