01804nas a2200217   4500008004100000022001400041245010000055210006900155260011000224300001400334490000800348520093200356100001801288700002301306700002701329700002701356700003401383700001901417700002501436856012501461       2016                        eng d  a0003-265400aImmobilization of multivalent glycoprobes on gold surfaces for sensing proteins and macrophages0 aImmobilization of multivalent glycoprobes on gold surfaces for s  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcFEB  a2250-22580 v1413 a<p>The multivalent display of carbohydrates on the cell surface provides cooperative binding to improve the specific biological events. In addition to multivalency, the spatial arrangement and orientation of sugars with respect to external stimuli also trigger carbohydrate-protein interactions. Herein, we report a non-covalent host-guest strategy to immobilize heptavalent glyco-beta-cyclodextrin on gold-coated glass slides to study multivalent carbohydrate-protein interactions. We have found that the localization of sugar entities on surfaces using beta-cyclodextrin (beta-CD) chemistry increased the avidity of carbohydrate-protein and carbohydrate-macrophage interactions compared to monovalent-beta-CD sugar coated surfaces. This platform is expected to be a promising tool to amplify the avidity of sugar-mediated interactions on surfaces and contribute to the development of next generation bio-medical products.</p>1 aGade, Madhuri1 aKhandelwal, Puneet1 aSangabathuni, Sivakoti1 aBavireddi, Harikrishna1 aMurthy, Raghavendra, Vasudeva1 aPoddar, Pankaj1 aKikkeri, Raghavendra  uhttps://library.ncl.res.in/content/immobilization-multivalent-glycoprobes-gold-surfaces-sensing-proteins-and-macrophages