02671nas a2200229   4500008004100000022001400041245010800055210006900163260011800232300001200350490000700362520177700369100002802146700001902174700001602193700002302209700002202232700002002254700001602274700002602290856012502316       2016                        eng d  a1466-502600aActinorectispora indica gen. nov., sp nov isolated from soil, a member of the family pseudonocardiaceae0 aActinorectispora indica gen nov sp nov isolated from soil a memb  aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcFEB  a939-9450 v663 a<p>The taxonomic positions of three Gram-stain-positive, aerobic strains, designated YIM 75722, 75726 and 75728(T), and isolated from a soil sample collected from Kurnool of Andhra Pradesh province, India, were assessed using a polyphasic approach. Growth was observed at pH 7.0-10.0 (optimum pH 7.0), 15-28 degrees C (optimum 28 degrees C) and 0-8% (w/v) NaCl (grew without NaCl). Strains showed cylindrical spores with straight-chain morphology on aerial mycelium, but did not reveal sporangium-like structures or fragmentation of the substrate mycelium. Whole-cell hydrolysates of all strains contained galactose and ribose as the diagnostic sugars and meso-diaminopimelic acid as the diamino acid. The predominant menaquinone was MK-9(H-4); MK-9 (H-6) and MK-10 (H-4) were present in smaller amounts. The phospholipid pattern consisted mainly of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. The major fatty acids were i-C-15: 0, ai-C-15: 0, i-C-17 : 0 and ai-C-17 : 0. The genomic DNA G+C content was 68.0 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain YIM 75728(T) should be placed within the family Pseudonocardiaceae, in which the strain formed a distinct lineage. The combination of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data support the conclusion that strain YIM 75728(T) represents a novel species of a novel genus of the family Pseudonocardiaceae for which the name Actinorectispora indica gen. nov., sp. nov., is proposed. Strain YIM 75728(T) (=DSM 45410(T)=CCTCC AA 209065(T)) is the type strain of Actinorectispora indica. Strain YIM 75728(T) was considered as the type strain over the other two strains based on the highest sequence read length of the strain.</p>1 aQuadri, Syed, Raziuddin1 aTian, Xin-Peng1 aZhang, Jing1 aRuwaili, Jamal, Al1 aHozzein, Wael, N.1 aAgsar, Dayanand1 aLi, Wen-Jun1 aDastager, Syed, Gulam  uhttps://library.ncl.res.in/content/actinorectispora-indica-gen-nov-sp-nov-isolated-soil-member-family-pseudonocardiaceae